1-(2-Fluoro-benzyl)-1H-pyrazolo[3,4-b]pyridine-3-carboxamidine

1- (2-fluorobenzyl) -1H-pyrazolo [3,4-b] pyridine-3-formamidine, the main application fields of this compound are quite extensive. In the field of pharmaceutical research and development, it may have unique pharmacological activities and can be used as potential drug lead compounds. The structure of Geiinpyrazolo-pyridine often has various biological activities, such as anti-tumor, anti-virus, anti-inflammatory, etc. In this compound, the introduction of 2-fluorobenzyl or the interaction between the compound and the biological target is changed, giving it specific pharmacological properties, allowing for drug creation for specific diseases.
In the field of pesticides, it may also emerge. Due to its structural properties, it may have inhibitory or killing effects on certain pests and pathogens. Under the general trend of green pesticide research and development, such novel compounds may become the focus of research and development, providing a new way for agricultural pest control, and is expected to meet the needs of environmental protection and sustainable development.
Furthermore, in the field of organic synthetic chemistry, it can be used as a key intermediate. With its unique molecular structure and functional groups, a series of organic compounds with more complex structures and more specific functions can be derived through a variety of organic reactions, contributing to the development of organic synthetic chemistry and expanding the structural diversity and functional diversity of organic compounds.

The synthesis of 1- (2-fluorobenzyl) -1H-pyrazolo [3,4-b] pyridine-3-formamidine is an important research direction in the field of organic synthesis. The following common synthesis paths are expressed in ancient Chinese.
Starting material, 2-fluorobenzyl halide and 1H-pyrazolo [3,4-b] pyridine-3-formonitrile are commonly selected as groups. First, the 2-fluorobenzyl halide is co-placed in an organic solvent with a suitable base, such as potassium carbonate, sodium carbonate, etc. The solvent can be selected from N, N-dimethylformamide (DMF), dimethyl sulfoxide (DMSO), etc. The temperature control is moderate, so that it undergoes nucleophilic substitution reaction, and the intermediate of 2-fluorobenzylation is obtained.
Then, the intermediate is reacted with an ammonia source, such as liquid ammonia, ammonia water or amine compounds, in the presence of a catalyst. The catalyst may be Raney nickel, palladium carbon, etc. Under specific temperature and pressure conditions, the cyano group is converted into amidine group. This step is the key to obtaining the target product. After the reaction is completed, the impurities are removed by separation and purification, such as column chromatography, recrystallization, etc., to obtain pure 1- (2-fluorobenzyl) -1H-pyrazolo [3,4-b] pyridine-3-formamidine.
Or there is another method, starting with 1H-pyrazolo [3,4-b] pyridine-3-carboxylic acid, first by esterification reaction to form the corresponding ester. Then 2-fluorobenzyl is introduced by nucleophilic substitution with 2-fluorobenzyl halide. Finally, with ammonia or amine reagents, through aminolysis reaction, the ester group is converted into amidine group, and the target product can also be obtained. After that, it still needs to be separated and purified to refine it.

1- (2-fluorobenzyl) -1H-pyrazolo [3,4-b] pyridine-3-formamidine is a kind of organic compound. Its physicochemical properties are particularly important, and it is related to the performance of this compound in various chemical processes and practical applications.
On its physical properties, under room temperature and pressure, 1- (2-fluorobenzyl) -1H-pyrazolo [3,4-b] pyridine-3-formamidine may be in the form of a solid state. Its appearance, or white to off-white powdery substance, is due to the regularity of its molecular structure and the interaction between molecules. Determination of its melting point can be a key indicator for identification and purity evaluation. Experimentally determined, the melting point value can be a specific range, which depends on the purity and crystallization of the compound.
As for solubility, 1- (2-fluorobenzyl) -1H-pyrazolo [3,4-b] pyridine-3-formamidine in organic solvents, such as dichloromethane, ethanol, etc., or exhibit a certain solubility. In polar organic solvent ethanol, due to the polar atoms such as nitrogen and oxygen in the molecule, it can form hydrogen bonds and other interactions with ethanol molecules, so it has moderate dissolution. However, in water, because of the existence of the hydrophobic part of the molecule, the solubility may be limited.
Another word about chemical properties, the pyrazolopyridine ring structure of 1- (2-fluorobenzyl) -1H-pyrazolo [3,4-b] pyridine-3-formamidine has aromatic properties, which makes its chemical properties relatively stable. However, the formamidine group, which is rich in nitrogen atoms and has lone pairs of electrons, is basic and can react with acids to form corresponding salts. Under appropriate conditions, due to the relative stability of benzyl carbon cation, the benzyl part of the compound can undergo nucleophilic substitution reaction, introduce other functional groups, and then derive various chemical products, providing many possibilities for the field of organic synthesis.

I do not know that 1- (2-fluorobenzyl) -1H-pyrazolo [3,4-b] pyridine-3-formamidine is in the market price range. This compound may be relatively niche, and its price often varies depending on purity, source, purchase volume, and market supply and demand. If you find a small amount of high purity from a chemical reagent supplier, it may reach hundreds or even thousands of yuan per gram. If you buy a large amount from a chemical raw material manufacturer, the price per gram may drop to tens of yuan due to scale effect. If you want to get an accurate price, you must consult the supplier in detail and compare their quotations to clarify the current market price.

1- (2-fluorobenzyl) -1H-pyrazolo [3,4-b] pyridine-3-formamidine, this substance is related to life, and it is essential to study the toxicity of safety and danger.
Considering the common sense, without detailed experiments and data, it is difficult to determine its safety and toxicity. However, depending on the chemical structure and the nature of similars, it may be possible to deduce a little.
This compound contains the nucleus of pyrazolo-pyridine, and such structures are common in many pharmacologically active molecules. Some with this structure can show biological activity, or it may be accompanied by adverse effects. The introduction of 2-fluorobenzyl, or the modification of its physicochemical and biological activities. Fluorine atoms have strong electronegativity, which can affect the polarity and lipophilicity of molecules, and then affect their absorption, distribution, metabolism and excretion in vivo.
As for safety, it involves many aspects. From the theory of toxicology, or acute toxicity, such as oral, percutaneous or inhalation, may affect the nervous, respiratory, circulatory and other systems of experimental animals. Subacute and chronic toxicity also need to be considered. Long-term exposure may cause organ damage, dysfunction, or even carcinogenesis, teratogenesis, and mutation.
Observe its chemical activity, or interact with biological macromolecules in the body, such as proteins, nucleic acids, etc. If the binding is improper, or disturb the normal physiological and biochemical processes, lead to adverse reactions. And its stability is different in different environments, or degradation of toxic substances.
In short, the safety and toxicity of 1- (2-fluorobenzyl) -1H-pyrazolo [3,4-b] pyridine-3-formamidine require rigorous experiments and multi-dimensional studies to determine its latent risk in biological systems and environments.